Cyanidin-3-O-β-glucoside Induces Lipoprotein Lipase Activity Following the Activation of AMP-Activated Protein Kinase in Skeletal Muscle Cells

doi:10.7506/spkx1002-6630-201305051

Abstract

Abstract: Cyanidin-3-O-β-glucoside (Cy-3-g)-rich foods have been reported to inhibit the onset of obesity, but the underlying mechanism has remained unclear. The induction of LPL activity in skeletal muscle will facilitate to the hydrolysis of triglyceride (TG) by β-oxidation, which can result in the decrease of TG store in adipose and the inhibition of obesity. In the present study, the isolation and culture method for skeletal muscle cells was established. The aim of our present study was to explore the effect of Cy-3-g on LPL activity in the skeletal muscle cells isolated from rats. The results demonstrated that Cy-3-g induced the activation of LPL in skeletal muscle cells due to the activation of pAMPK, suggesting that Cy-3-g may have a potential role against obesity through the regulation of lipid metabolism.

Key words: cyanidin-3-O-β-glucoside, skeletal muscle cells, lipoprotein lipase, AMP-activated protein kinase

CLC Number:

R151.1

WEI Xiao-yi1，BAI Chen1,*，TANG Li-wei1，LU Hong2，ZHANG Jing-wei1. Cyanidin-3-O-β-glucoside Induces Lipoprotein Lipase Activity Following the Activation of AMP-Activated Protein Kinase in Skeletal Muscle Cells[J]. FOOD SCIENCE, 2013, 34(5): 246-250.

References

[1] KUHNAU J. The flavonoids. A class of semi-essential food components: their role in human nutrition[J]. World review of nutrition and dietetics, 1976, 24:117-191.
[2] TSUDA T, HORIO F, KITOH J, et al. Protective effects of dietary cyanidin 3-O-beta-D-glucoside on liver ischemia-reperfusion injury in rats[J]. Arch Biochem Biophys, 1999, 368(2):361-366.
[3] XIA M, HOU M, ZHU H, et al. Anthocyanins induce cholesterol efflux from mouse peritoneal macrophages: the role of the peroxisome proliferator-activated receptor {gamma}-liver X receptor {alpha}-ABCA1 pathway[J]. The Journal of biological chemistry, 2005, 280(44):36792-36801.
[4] XU JW, IKEDA K, YAMOTI Y. Upregulation of endothelial nitric oxide synthase by cyanidin-3-glucoside, a typical anthocyanin pigment[J]. Hypertension, 2004, 44(2):217-222.
[5] LI SQ, ZHANG QH. Advances in the development of functional foods from buckwheat[J]. Crit Rev Food Sci Nutr, 2001, 41(6):451-464.
[6] XIA M, LING WH, MA J, et al. Supplementation of diets with the black rice pigment fraction attenuates atherosclerotic plaque formation in apolipoprotein e deficient mice[J]. The Journal of nutrition, 2003, 133(3):744-751.
[7] XIA X, LING W, MA J, et al. An anthocyanin-rich extract from black rice enhances atherosclerotic plaque stabilization in apolipoprotein E-deficient mice[J]. The Journal of nutrition, 2006, 136(8):2220-2225.
[8] SASAKI R, NISHIMURA N, HOSHINO H, et al. Cyanidin 3-glucoside ameliorates hyperglycemia and insulin sensitivity due to downregulation of retinol binding protein 4 expression in diabetic mice[J]. Biochem Pharmacol, 2007, 74(11):1619-1627.
[9] GUO H, LING W, WANG Q, et al. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. indica) on hyperlipidemia and insulin resistance in fructose-fed rats[J]. Plant Foods Hum Nutr, 2007, 62(1):1-6.
[10] QIN Y, XIA M, MA J, et al. Anthocyanin supplementation improves serum LDL- and HDL-cholesterol concentrations associated with the inhibition of cholesteryl ester transfer protein in dyslipidemic subjects[J]. The American journal of clinical nutrition, 2009, 90(3):485-492.
[11] TSUDA T, HORIO F, UCHIDA K, et al. Dietary cyanidin 3-O-beta-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice[J]. The Journal of nutrition, 2003, 133(7):2125-2130.
[12] BESSESEN DH, RUPP CL, ECKEL RH. Trafficking of dietary fat in lean rats[J]. Obes Res, 1995, 3(2):191-203.
[13] ZECHNER R. The tissue-specific expression of lipoprotein lipase: implications for energy and lipoprotein metabolism[J]. Curr Opin Lipidol, 1997, 8(2):77-88.
[14] 卫晓怡，白晨，唐立伟等. 矢车菊素-3-葡萄糖苷通过AMPK抑制成熟脂肪细胞脂蛋白脂酶活性[J]. 食品科学，2012. （已录用）
[15] REEVES PG, NIELEN FH, FAHEY GC, et al. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet[J]. The Journal of nutrition, 1993, 123(11):1939-1951.
[16] LEONHARDT U, RITZEL U, OTTLEBEN M, et al. Circulating human hepatic lipase mRNA in patients with hepatocellular carcinoma and healthy controls[J]. The European journal of surgery, 1999, 165(6):539-542.
[17] FAN C, YAN J, QIAN Y, et al. Regulation of lipoprotein lipase expression by effect of hawthorn flavonoids on peroxisome proliferator response element pathway[J]. J Pharmacol Sci, 2006, 100(1):51-58.
[18] KERN PA. Potential role of TNFalpha and lipoprotein lipase as candidate genes for obesity[J]. The Journal of nutrition, 1997, 127(9):1917-1922.
[19] GREENWOOD MR. The relationship of enzyme activity to feeding behavior in rats: lipoprotein lipase as the metabolic gatekeeper[J]. International journal of obesity, 1985, 9 Suppl 1:67-70.
[20] GOLDBERG IJ, MERKEL M. Lipoprotein lipase: physiology, biochemistry, and molecular biology[J]. Front Biosci, 2001, 6: 388-405.
[21] PYKALISTO OJ, SMITH PH, BRUNZELL JD. Determinants of human adipose tissue lipoprotein lipase. Effect of diabetes and obesity on basal- and diet-induced activity[J]. The Journal of clinical investigation, 1975, 56(5):1108-1117.
[22] GRUEN R, HIETANEN E, GREENWOOD MR. Increased adipose tissue lipoprotein lipase activity during the development of the genetically obese rat (fa/fa) [J]. Metabolism: clinical and experimental, 1978, 27(12 Suppl 2):1955-1966.
[23] SCHWARTZ RS, BRUNZELL JD. Increased adipose-tissue lipoprotein-lipase activity in moderately obese men after weight reduction[J]. Lancet, 1978, 1(8076):1230-1231.
[24] ONG JM, KERN PA. Effect of feeding and obesity on lipoprotein lipase activity, immunoreactive protein, and messenger RNA levels in human adipose tissue[J]. The Journal of clinical investigation, 1989, 84(1):305-311.
[25] BESSESEN DH, ROBERTSON AD, ECHEL RH. Weight reduction increases adipose but decreases cardiac LPL in reduced-obese Zucker rats[J]. The American journal of physiology, 1991, 261(2):246-251.
[26] AGUILERA CM, GIL-CAMPOS M, CANETE R, et al. Alterations in plasma and tissue lipids associated with obesity and metabolic syndrome[J]. Clin Sci (Lond), 2008, 114(3):183-193.
[27] SAHA AK, RUDERMAN NB. Malonyl-CoA and AMP-activated protein kinase: an expanding partnership[J]. Mol Cell Biochem, 2003, 253(1-2):65-70.
[28] AN D, PULINIKUNNIL T, QI D, et al. The metabolic "switch" AMPK regulates cardiac heparin-releasable lipoprotein lipase[J]. American journal of physiology, 2005, 288(1): 246-253.