Comparison of Three Detectors Used for HPLC Determination of Monoglycerides

doi:10.7506/spkx1002-6630-201308033

Abstract

Abstract: High performance liquid chromatography-ultraviolet detector(HPLC-UV), high performance liquid chromatography-differential refractive index detector(HPLC-RI) and high performance liquid chromatography- evaporative light scattering detector(HPLC-ELSD) were compared to measure the content of monoglycerides in emulsions.When using UV detector, the result was seriously influenced by impurities, the signal of the samples was weak and the sensitivity was low. RI detector could had a good linear relationship only in the higher concentration range, so it was not suitable for measuring low content of monoglycerides. When using ELSD detector, the linear range of monoglycerides were between 0.040~5.000 mg/mL. The correlation coefficient of ELSD was 0.9998 , the recovery rate of monoglycerides reached 92.94%, the standard deviations was 2.24 and the variation coefficients was 2.41%. HPLC-ELSD method was sensitive, accurate and reproducible and may therefore be applicable for the determination of monoglycerides in emulsions .

Key words: monoglyceride, content determination, HPLC-UV, HPLC-RI, HPLC-ELSD

CLC Number:

TS252.7

FANG Min，ZHAO Mou-ming，ZHAO Qiang-zhong. Comparison of Three Detectors Used for HPLC Determination of Monoglycerides[J]. FOOD SCIENCE, 2013, 34(8): 161-163.

References

[1]VEREECKEN J, MEEUSSEN W, LESAFFER A, et al.Effect of water and monoglycerides concentration on the behaviour of monoglycerides containing fat systems[J].Food research international,2010,43(3):872-881
[2]张万福.[J].劳国强食品乳化剂北京中国轻工业出版社,1993,:-
[3]胡永涛, 刘钟栋, 杨菁等.单甘酯、甘二酯高纯品的生产、理化性质及特殊用途[J].中国食品添加剂,2009,(1):57-64
[4]MOHAREB A, THEVENON M F, WOZNIAK E, et al.Effects of monoglycerides on leachability and efficacy of boron wood preservatives against decay and termites[J].International Biodeterioration & amp: Biodegradation,2010,64(2):135-138
[5]INGRID S, HELENA L W.Phase properties and structure of a monoglyceride/sucrose/water system[J].Chemistry and Physics of Lipids,1990,55(2):97-101
[6]栾霞, 樊铁.高碘酸氧化法测定油脂中1_单甘酯的含量[J].中国油脂,2008,33(9):73-75
[7]倪永全, 杜志云.单甘酯的高效液相色谱分析[J].无锡轻工大学学报,1996,15(1):59-61
[8]LOPEZ A, CASTELLOTE A I, SABATER M C.Direct determination by high-performance liquid chromatography of sn-2 monopalmitin after enzymatic lipase hydrolysis[J].Journal of Chromatography B: Biomedical Sciences and Applications,2001,760(1):97-105
[9]李长秀, 唐忠, 杨海鹰.气相色谱法测定生物柴油样品中脂肪酸甲酯和脂肪酸甘油酯的含量[J].分析测试学报,2005,24(5):66-68
[10]冯月琴, 杨宏杰.气相色谱法测定月桂酸单甘酯含量的方法研究[J].中国食品学报,2005,5(1):59-63
[11]王丽丽, 汪勇, 胡长鹰等.高温气相色谱分析甘油酯的研究[J].中国油脂,2011,36(7):75-79
[12]MATSUMIYA K, TAKAHASHI W, INOUE T, et al.Effects of bacteriostatic emulsifiers on stability of milk-based emulsions[J].Journal of Food Engineering,2010,96(2):185-191
[13]张英, 石雪萍, 张卫民.HPLC-ELSD法与HPLC-RID法检测蜂蜜中糖分的比较[J].中国野生植物资源,2009,28(1):43-47
[14]SAWA K.nullINOUE S, LYSENKO E, et al. Effects of purified monoglycerides on Canadian short process and sponge and dough mixing properties, bread quality and crumb firmness during storage[J].. Food Chemistry,2009,:-
[15]黄海时, 汪晟, 康超等.HPLC-ELSD法测定维生素D3和光转化异构体[J].食品工业科技,2010,31(05):351-355