Rapid Detection of Campylobacter jejuni in Foods by PCR-ELISA

doi:10.7506/spkx1002-6630-201310054

Abstract

Abstract:

A PCR-ELISA method was developed to detect Campylobacter jejuni contamination in foods through the combined use of PCR, nucleic acid hybrid reaction and enzyme coloration. A forward PCR primer and a probe labeled with biotin at the 5’ end was designed from Campylobacter jejuni 16S rRNA gene sequence by using Primer Express Software 3.0. The testing conditions were optimized and developed for PCR-ELISA detection of Campylobacter jejuni in foods. The PCRELISA method revealed 10 times higher sensitivity than the ordinary PCR method. PCR-ELISA has potential applications in such fields as clinic diagnosis, food hygiene monitoring and pathogen detection in food imports and exports.

Key words: Campylobacter jejuni, polymerase chain reaction-enzyme linked immunosorbent assay, detection

CLC Number:

TS201.25

SHI Yan-yu，LIU Jin-hua，XUE Li-gang，BING Wei，HUA Lei，ZHAO Li-qun，ZHOU Liang，LIU Xiao-hui，WANG Xiao，ZHANG Qing-bo，LENG Xi-fang. Rapid Detection of Campylobacter jejuni in Foods by PCR-ELISA[J]. FOOD SCIENCE, 2013, 34(10): 246-249.

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