Determination of of the Herbicide Metolachlor by Enzyme-Linked Immunosorbent Assay

doi:10.7506/spkx1002-6630-201310027

Abstract

Abstract:

Using one step reaction, metolachlor was modified with 3-marcapropanoic acid (3-MPA) to obtain the hapten
3-(2-((2-ethyl-6-methylphenyl)(1-methoxypropan-2-yl)amino)-2-oxoethylthio)propanoic acid (MMPA), which was then
coupled to bovine serum albumin (BSA) as immunogen (MMPA-BSA) and ovalbumin (OVA) as coating antigen (MMPAOVA)
using active ester method, respectively. New Zealand rabbits were immunized with MMPA-BSA. Based on the
obtained polyclonal antibody, an indirect competitive ELSIA (icELISA) was developed successfully for the detection of
metolachlor and demonstrated excellent performance. The 50% inhibition concentration (IC50) was 34.3 ng/mL and the
detectable range (IC20–IC80) was 12.3–99.2 ng/mL, while the limit of detection (LOD, IC10) was 6.3 ng/mL. The antibody
showed low cross-reactivity (10.9%) towards S-(-)-metolachlor. However, no significant cross-reactivity towards other
structurally related compounds was found. The recoveries of spiked samples were in the range of 89.5%–107.9% with RSD
ranging from 9.2% to 14.5%. Our results indicated that the proposed icELISA method is highly specific, sensitive, accurate
and suitable for the rapid detection of herbicide metolachlor in water samples.

Key words: metolachlor, enzyme-linked immuno sorbent assay (ELISA), antibody

CLC Number:

TS201.6

ZHANG Chi-jian，WANG Qiang，TANG Qiu-shi，LIU Ying-ju，SUN Yuan-ming，TANG Yun-jian，LEI Hong-tao. Determination of of the Herbicide Metolachlor by Enzyme-Linked Immunosorbent Assay[J]. FOOD SCIENCE, 2013, 34(10): 126-129.

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