Effect of Wheat Peptide on the Nitrogen Metabolism and Gastrointestinal Mucosal Structure of Rats

doi:10.7506/spkx1002-6630-201305055

Abstract

Abstract: Purpose: The present study was aimed to investigate the potential effect of the wheat peptide on the nitrogen metabolism, gastrointestinal morphology, and enzyme activity of small intestine mucosa in rats. Methods: 50 SD rats were randomly divided into control group, low, medium and high doses of the wheat peptide group and wheat gluten group. The animals were daily administered with water, wheat peptide or wheat protein through ig route for continuous 30 days. The feces and urine samples of rats in 24 hours were collected using metabolic cages on 10th, 20th, 30th day. On 30th days, rats were sacrificed and serum, stomach and small intestine mucosa were harvested to investigate the changes of the indicators of nitrogen metabolism, serum biochemical parameters, surface scanning electron microscopy of gastrointestinal and enzyme activity of small intestinal mucosa. Results: Following 30 days of treatment, low dose of wheat peptide can significantly increase the rat protein digestibility, nitrogen deposition, net protein utilization and the protein biological values were also enhanced by low- and mid-dose of wheat peptide administration. Wheat peptide at all given doses was able to increase the serum total protein content. The gastrointestinal tract epithelial cells of rat following feeding low and medium dose of wheat peptide were more fully lined, tightly connected, neat and orderly than those of control group. Low, middle and high dose wheat peptide could also increase the aminopeptidase enzyme activity of intestinal mucous membrane and high doses of wheat peptide was able to enhance the Na+-K+-ATPase activity of intestinal mucous membrane. Conclusion: a certain dose of wheat peptide can improve the protein absorption and utilization of rats and the mechanisms might be related to its benefits in promoting the growth of epithelial cells of the gastrointestinal tract and its capacity to enhance the aminopeptidase and Na+-K+-ATPase activities of small intestinal mucosa.

Key words: wheat peptide, nitrogen metabolism, gaster & small intestine-morph, aminopeptidase, Na+-K+-ATPase

CLC Number:

S858

PAN Xing-chang1，YIN Hong2，GU Rui-zeng1，XU Ya-guang1，CAI Mu-yi1，SUN Gui-ju2. Effect of Wheat Peptide on the Nitrogen Metabolism and Gastrointestinal Mucosal Structure of Rats[J]. FOOD SCIENCE, 2013, 34(5): 264-269.

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