Macroporous Resin Purification and Composition Analysis of Polyphenols from Blueberry Leaves

doi:10.7506/spkx1002-6630-201310019

Abstract

Abstract:

Adsorption and desorption of 8 types of macroporous resins towards polyphenols from blueberry leaves were
investigated. The adsorption capacity and desorption rate of HPD400 resin was 52.95 mg/g and 95.96% respectively, and the
time to reach equilibrium between adsorption and desorption was short, so it was the most suitable resin to purify blueberry
leaves polyphenols. When 140 mL of 3 mg/mL samples with pH 2 was flowed through the HPD400 resin at a speed of 1
mL/min, the adsorption reached dynamic equilibrium. The adsorbed polyphenols could be completely eluted with 80 mL of
40% ethanol at 1 mL/min, resulting in a desorption rate of 90.50%. After being purified with HPD400 resin, the purity of
polyphenols was increased from 38.75% to 69.38%. HPLC-DAD-MS analysis showed that the blueberry leaf polyphenols
were rich in caffeoylquinic acids and quercetin glycosides.

Key words: blueberry leaves polyphenols, macroporous resin, purification, HPLC-DAD-MS

CLC Number:

TS201.1

FENG Jin，LI Min，ZENG Xiao-xiong，LI Chun-yang. Macroporous Resin Purification and Composition Analysis of Polyphenols from Blueberry Leaves[J]. FOOD SCIENCE, 2013, 34(10): 86-91.

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