Determination of Benzoylurea Insecticides in White Grape Juice by High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201310036

Abstract

Abstract:

Residues of benzoylurea insecticides (teflubenzuron, triflumuron, diflubenzuron and hexaflumuron) in white
grape juice were determined by dispersive liquid-liquid micro-extraction combined with normal-phase high performance
liquid chromatography. In the dispersive liquid-liquid micro-extraction, the extraction solvent was methylene chloride and
dispersive solvent was methanol. The analytes were separated on Apollo Silica column with a mobile phase made up of
n-hexane and anhydrous alcohol (97:3, V/V) at a flow rate of 1.0 mL/min and detected at 200 nm. Good linearity was found
in the range of 0.33 to 100 μg/mL with correlation coefficients above 0.999. The limits of detection were between 5.80 ng/mL and
6.60 ng/mL. The average recoveries of the benzoylurea insecticides ranged from 73.72% to 97.85%, with relative standard
deviations between 1.33% and 4.62%. The proposed method is rapid and highly reproducible and shows a limit of detection.
Therefore it is applicable for the determination of benzoylurea insecticide residues in white grape juice.

Key words: high performance liquid chromatography (HPLC), benzoylurea, dispersive liquid-liquid micro-extraction, white grape juice

CLC Number:

O657.7

ZHOU Jian-ke，HAN Chao-jia，TANG Cui-ling，FU Yan-fang. Determination of Benzoylurea Insecticides in White Grape Juice by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2013, 34(10): 169-172.

References

[1] 吴刚，吴俭俭，赵珊红，等．固相萃取-液相色谱分析蔬菜中多种苯甲酰脲类昆虫生长调节剂残留量[J]．检验检疫学刊，2009，19(1)：10-13．

[2] 刘锦霞，王美玲，黄志强，等．高效液相色谱-串联质谱法测定猪肉中10种苯甲酰脲类杀虫剂[J]．分析实验室，2010，29(9)：39-42．

[3] 丁慧瑛，谢文，周召千，等。蔬菜中11种苯甲酰脲类农药残留的液相色谱-串联质谱法测定[J]．分析测试学报，2009，28(8)：970-974．

[4] 韩笑，娄喜山，张莉，等．基质固相分散-超高效液相色谱-串联质谱法检测蔬菜中残留的苯甲酰脲类和双酰肼类杀虫剂[J]．色谱，2010，28 (4)：341-347．

[5] Sannino A，Bandini M．Determination of seven benzoylphenylurea insecticides in processed fruit and vegetables using high performance liquid chromatography／tandem mass spectrometry[J]．Rapid Commun Mass Spectrom，2005，19(19)：2729-2733．

[6] Miliadis G E，Tsiropoulos N G，Aplada-Sarlis P G．High performance liquid chromatographic determination of benzoylurea insecticides residues in grapes and wine using liquid and solid-phase extraction[J]．Journal of chromatography A，1999，835(1/2)：113-120．

[7] Leandro C C，Hancock P，Fussell R J，et a1．Ultra-performance liquid chromatography for the determination of pesticide residues in foods by tandem quadrupole mass spectrometry with polarity switching[J]．Journal of chromatography A，2007，1144(2)：161-169．

[8] 刘世勤，李方实．高效液相色谱法测定水体中的苯甲酰脲类杀虫剂残留量[J]．南京大学学报，2010，32(1)：102-105．

[9] 周建科，杨冬霞，徐鹏．微萃取／反相液相色谱法测定水中苯甲酰脲类农药[J]．中国给水排水，2011，27 (8)：96-98．

[10] Garrido-Frenich A，Gil-Garcia M D，Arrebola F J，et a1．Determination of parts per trillion level of benzoylurea pesticides in groundwater by high performance liquid chromatography-electrospray ionization mass spectrometry[J]．Chromatographia，2000，52(9/l0)：569-574．

[11] 王连珠，周昱，陈泳，等．果蔬中8种苯甲酰脲类农药残留量的液相色谱-串联质谱法测定[J].分析测试学报，2010，29 (3)：289-293．

[12] 周建科，刘瑞英，宋歌，等．液下单清微萃取-高效液相色谱法测定果汁中苯甲酰脲农药残留[J]．食品科技，2009，34(8)：290-293．

[13] Rezaee M，Assadi Y， Milani Hosseini M R， et a1．Determination of organic compounds in water using dispersive liquid–liquid microextraction[J]．Journal of Chromatography A，2006，1116(1/2)：1-9．

[1]	WANG Shuhui, SONG Shasha, CAO Xueli. Application of Discriminant Analysis and Similarity Evaluation of HPLC Fingerprints of Flavor Components in Xinyang Maojian Tea Grading [J]. FOOD SCIENCE, 2019, 40(8): 180-185.
[2]	ZHANG Zidong, FU Dongmei, ZHANG Weipeng, ZHANG Lin, ZU Yuangang. Simultaneous Determination of Five Phenylpropanoids in Eucommia ulmoides Oliv. from Different Ages and Parts by HPLC [J]. FOOD SCIENCE, 2019, 40(8): 186-191.
[3]	LI Jiaxiu, ZHANG Chunling, LIU Hui, CHEN Dalei, LIU Jiechao, JIAO Zhonggao. Fingerprinting of Organic Acids by High Performance Liquid Chromatography for Authentication of Sweet Cherry Juice [J]. FOOD SCIENCE, 2019, 40(16): 327-334.
[4]	DENG Junlin, ZHU Yongqing, XIA Chen, CHEN Jian, ZHAO Xuzhu, ZHANG Yingjiao, LI Huajia, DENG Haiyun, LI Juan. Phenolic Contents and Antioxidant Activity in Methanol Extracts from Gynostemma pentaphyllum (Thumb.) Makino [J]. FOOD SCIENCE, 2019, 40(14): 265-269.
[5]	XU Mingfang, SHEN Linyan, YANG Yunshu, FU Lijun, SUN Yong, WANG Yangyang, PENG Lu, HUANG Xiaojing, LI Yan. Comparison of HPLC-MS/MS and HPLC for the Synchronous Determination of Ergosterol and VD2 in White Hypsizygus marmoreus [J]. FOOD SCIENCE, 2019, 40(12): 237-244.
[6]	CHENG Lijuan, LIU Guishan, HE Jianguo, YANG Xiaoyu, WAN Guoling, ZHANG Chong, MA Chao. Nondestructive Detection of Sucrose Content of Lingwu Changzao Jujubes by Hyperspectral Imaging [J]. FOOD SCIENCE, 2019, 40(10): 285-291.
[7]	YANG Chunxiang, WANG Yifen, WANG Jing, LI Li, WEN Yihua, YANG Binghao. High Performance Liquid Chromatography Method for Rapid Detection of Carvacrol and Thymol in Active Food Packaging Film [J]. FOOD SCIENCE, 2018, 39(16): 317-320.
[8]	FAN Zirui, HU Jiafei, JIANG Ruini, YANG Xingling, JIN Huoxi. Evaluation of Cysteine Dioxygenase Activity in Marine Organisms by High Performance Liquid Chromatography [J]. FOOD SCIENCE, 2018, 39(12): 174-178.
[9]	YANG Xiaohui, SHI Guangbo, BAI Xiaobin, ZHAO Chenxuan, LI Bosheng. Flavonoid Content and Antibacterial Activity of Xanthoceras sorbifolia Bunge Fruit Drop [J]. FOOD SCIENCE, 2018, 39(10): 53-58.
[10]	ZHANG Wei, ZHANG Yong, YIN Zhenhua, KANG Wenyi. Determination of the Active Ingredients in Cultured Cordyceps militaris by Ultrasound-Assisted Ionic Liquid Microextraction Coupled with Reversed Phase Liquid Chromatography [J]. FOOD SCIENCE, 2017, 38(8): 179-183.
[11]	CHEN Xia, WANG Bo, YE Rong, LI Min, ZHOU Jigen, ZHANG Bo, HAN Shunyu. Optimization of Solvent Induced Phase Transition Extraction of Patulin from Aqueous Matrix by Response Surface Methodology for HPLC Analysis [J]. FOOD SCIENCE, 2017, 38(6): 215-220.
[12]	DUAN Kaili, GAO Mingjing, LIU Yongquan, ZHANG Tingting, CHEN Li, ZHAO Sanzhong, LUO Liping. Phenolic Compounds and Essential Oil Compositions of Artemisia argyi Leaves from Two Growing Areas [J]. FOOD SCIENCE, 2017, 38(4): 204-210.
[13]	LAI Guoyin, LIN Jianzhong, ZENG Qi, LIN Liyi, DING Yinan, ZHANG Zhigang, XU Dunming. Simultaneous Determination of Sodium (or Calcium) Propionate and Sodium Diacetate in Oyster Sauce and Other Similar Seasonings by High Performance Liquid Chromatography Using Kjeldahl Distillation Apparatus [J]. FOOD SCIENCE, 2017, 38(4): 270-274.
[14]	Long Xiaoqin, Tang jie, Zhao Jingfang, Zeng Fankun. Ultrasonic-Assisted Enzymatic Extraction and HPLC Analysis of Flavonoids from Old Leaves of Toona sinensis [J]. FOOD SCIENCE, 2017, 38(22): 256-262.
[15]	LIU Huilin, NI Tianhong, ZHOU Kaiwen, WANG Jing. Molecularly Imprinted Solid-Phase Extraction Coupled with High Performance Liquid Chromatography for the Detection of Dicyandiamide in Milk Powder [J]. FOOD SCIENCE, 2017, 38(20): 203-208.