FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (10): 255-258.doi: 10.7506/spkx1002-6630-201310056

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Determination of Olaquindox Metabolite Residues in Fishery Products by High Performance Liquid Chromatography

BEI Yi-jiang，WANG Yang，HE Feng，REN Sheng-lan   

  1. 1. Aquatic Product Quality Testing Center of Zhejiang Province, Hangzhou 310012, China；
    2. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China
  • Received:2012-02-16 Revised:2013-03-31 Online:2013-05-25 Published:2013-05-07
  • Contact: Jiang YiBei E-mail:zsbij@163.com

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Abstract:

A high performance liquid chromatography method was established to determine olaquindox metabolite
(3-methyl-2-quinoxalinecarboxylic acid, MQCA) residues in fishery products in this study. Olaquindox metabolite
residues were extracted with 2 mol/L hydrochloric acid, and purified on an SPE column. Sample analysis was performed
using RP-HPLC and external standard method. In the concentration range of 0.01 to 1.0 μg/mL, an excellent linearity
was observed with a correction coefficient of 0.9998. The LOD (limit of detection) was 4.0 μg/kg and LOQ (limit of
quantification) was 12.0 μg/kg. Average recovery rates for different species of fishery products spiked at three levels
varied between 71.0% and 86.5% with a RSD of 3.84% to 7.32%. The recovery of MQCA was increased by an average
of 6.4% under optimized pretreatment conditions with more complicated extraction procedure and shortened oscillation
time. Moreover, an average increase of 13.0% in MQCA recovery was obtained by omitting filtration.

Key words: high performance liquid chromatography, fishery product, olaquindox metabolite residues