Simultaneous Determination of 3 Tetracycline Residues in Chicken Tissues by High-Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201310039

Abstract

Abstract:

A simultaneous high performance liquid chromatography (HPLC) method was developed for the determination
of 3 tetracycline residues in chicken tissues. Chicken tissue samples were extracted with citrate buffer (pH 4) and 20% TCA,
and clean-up on C-18E solid phase (SPE) cartridge. Chromatographic separation was performed on a C18 column with a mobile
phase consisting of acetonitrile and methanol and oxalic acid at a flow rate of 1.0 mL/min by gradient elution. Detection
wavelength was 351 nm and external standard method was used for quantification. The calibration curves of 3 tetracyclines
exhibited good linearity with correlation coefficients of 0.99. The average recovery rate for 3 tetracyclines in chicken tissues
spiked at 500 μg/kg varied from 86.2% to 89.3%, with relative standard deviations between 0.854% and 1.213% (n = 10).
The limit of quantification was 10 μg/kg.

Key words: chicken tissue, tetracycline, oxytetracycline, chlorotetracycline, high performance liquid chromatography

CLC Number:

TS207.3 A

GE Yun-zhi，YU Xiao-bo，ZHOU Guang-hong*，XU Xing-lian. Simultaneous Determination of 3 Tetracycline Residues in Chicken Tissues by High-Performance Liquid Chromatography[J]. FOOD SCIENCE, 2013, 34(10): 180-183.

References

［1］TSAI W H，HUANG T C，CHEN H H，et al. Determination of tetracyclines in surface water and milk by the magnesium hydroxide coprecipitation method[J]. Journal of Chromatography A，2010，1217(3)：415-418．

［2］2377/90/EC:Council Regulation (EEC) of 26 June 1990 laying down a Community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin，Brussels，Belgium，Off. J. Eur.Commun[S]. 1990，L224，12124．

［3］U. S. Code of Federal Regulations，Title 21，Part 558，Sections 128 and 450. U.S.Government Printing Office，Washington DC[S].2003，(Chapter 1)．

［4］Sarmah A K，Meyer M T，Boxall A B A．A global perspective on the use，sales，exposure pathways，occurrence，fate and effects of veterinary antibiotics (VAs) in the environment［J］．Chemosphere，2006，65(5)：725-759．

［5］ Zhao L，Dong Y H，Wang H． Residues of veterinary antibiotics in manures from feedlot livestock in eight provinces of China［J］．Science of the Total Environment，2010，408(5)：1069-1075．

［6］FANG Guozhen，HE Jinxing，WANG Shuo. Multiwalled carbon nanotubes as sorbent for on-line coupling of solid-phase extraction to high-performance liquid chromatography for simultaneous determination of 10 sulfonamides in eggs and pork [J]. Journal of Chromatography，2006，1127(1/2)：12-17．

［7］Scheer M. Studies on the Antimicrobial Agents against Five．Pathogaents Isolated in Calves in the Netherlands[J]. Vet Quart，1990，12(4)：212-219．

［8］陆孙琴，李轶，黄晶晶，等.[J]. 环境科学，2011，11(32)：3419-3424．

［9］刘兴泉，冯震，姚蕾，等. 采用高通量微生物法和HPLC法检测猪肉中四环素和磺胺类抗生素残留[J]. 食品与发酵工业，2001，4(37)：194-197．

［10］黄晓蓉，郑晶，吴谦，陈彬，汤敏英.食品科学[J]，2007，28(8)：418．

［11］秦燕，陈毓芳，朱柳明. 动物肌肉中四环素族药物多残留的固相萃取-酶联免疫分析[J]. 中山大学学报，2004，1(43)：84-86．

［12］林杰，黄晓蓉，郑晶，等. 放射免疫法快速检测猪尿样中的四环素类药物残留[J]. 饲料工业，2009，15(30)：45-47．

［13］陈丽，潘文庆，马晓康. 5种四环素类抗生素快速鉴别方法的建立[J].中国药师，2010，6(13)：893-894．

［14］Samanidou V F，Nikolaidou K I，Papadoyannis I N. Development and validation of an HPLC confirma-tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC [J]Journal of Separation Science，2005，28(17)：2247-2258．

［15］Ueno R，Sangrungruang K，Miyakawa M. A Simp lified Method for the Determination of Several Fish Drugs in Edible Fish and Shrimp by High performance Liquid Chromatography[J].Food Research International，1999，32(9)：629-633．

［16］贺蕴普，贺德春，王志良，等. 养殖废水中四环素HPLC分析方法的研究[J].广州化学，2011，1(36)：26-30．

［17］GB/T 14931. 畜禽肉中土霉素、四环素、金霉素残留量测定[S]．

［18］李俊锁，邱月明，王超. 兽药残留分析[M]. 上海:上海科学技术出版社，2002：365．

［19］岳振凤，郑卫平，谢丽琪等. 高效液相色谱法测定动物组织中四环素类抗生素残留量的研究[J]. 分析试验室，2006，25(10)：24-28．

［20］叶英. 高效液相色谱法测定畜禽肉中土霉素、四环素、金霉素残留量[J]. 热带农业工程，2003，31(1)：25-27．

［21］SN/T0005-1996 出口商品中农药、兽药残留量及生物毒素检验方法标准编写的基本规定[S]．

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