Optimization of Extraction Process for Total Alkaloids from Stems and Leaves of Fritillaria cirrhosa D. Don by Response Surface Methodology

doi:10.7506/spkx1002-6630-201310003

Abstract

Abstract:

The extraction of total alkaloids from aboveground parts (stems and leaves) of Fritillaria cirrhosa D. Don was
optimized. One-factor-at-a-time design was employed to identify four main process parameters. The selected variables were
optimized by response surface analysis based on the extraction yield of total alkaloids. The optimal extraction parameters were
soaking in aqueous ammonia for 4 h and subsequent extraction at 74 ℃ for 6.5 h with a solvent-to-material ratio 31:1. The
extraction yield of total alkaloids obtained under these conditions was 2.785 mg/g. Furthermore, the content of total alkaloids
in stems and leaves of wild Fritillaria cirrhosa D. Don was much higher than in those of cultivated Fritillaria cirrhosa D.
Don. Leaves of wild Fritillaria cirrhosa D. Don had the highest total alkaloid content of 4.998 mg/g. The described method is
reliable, highly effective and suitable for the extraction of total alkaloids from stems and leaves of Fritillaria cirrhosa D. Don.

Key words: Fritillaria cirrhosa, stem and leaf, total alkaloids, extraction

CLC Number:

TS201.1

ZHANG Jian-guang，XU Yan，LIU Yuan，Lü Lu-yang，ZENG Rui，ZHANG Zhi-feng*. Optimization of Extraction Process for Total Alkaloids from Stems and Leaves of Fritillaria cirrhosa D. Don by Response Surface Methodology[J]. FOOD SCIENCE, 2013, 34(10): 11-15.

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