FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (2): 36-41.

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Lipase-Catalyzed Synthetic of Diacylglycerol of Damellia oil and its Fatty Acid change

  

  • Received:2012-07-09 Revised:2012-12-20 Online:2013-01-25 Published:2013-01-15

Abstract: Diacylglycerol (DAG) was prepared through lipase-catalyzed reaction between camellia oil and glycerol in the solvent-free system using immobilized enzyme catalytic. Lipozyme RMIM immobilized lipase was selected as the catalyst, substrate ratio, enzyme dosage, reaction temperature, reaction time and other factors were considered to investigating the yield of the catalytic reaction products, both single factor experiments and response surface optimization method were used. The best conditions were: substrate ratio (glycerol:camellia oil, mol:mol) is 1:1.7, the enzyme concentration was 10.7% (wt%), reaction temperature of 63℃, the reaction time of 13.5 h. Purity of DAG were close to 99.7% after molecular distillation purification. Fatty Acid composition of both DAG oil and camellia oil were detected by the gas chromatographic, the main fatty acids composition of DAG oil are C16:0, C18:1 and C18:2, and no significant difference from camellia oil was detected. As a result, the camellia DAG oil remains the same fatty acid composition of camellia oil, as well as possesses the functional features of the diacylglycerol.

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