Optimization of Successive Extraction of Saponins, Flavonoids and Polysaccharides from Gynostemma pentaphyllum Stems and Leaves

Abstract

Abstract: This paper reports the application of orthogonal array design method to optimize the successive extraction of saponins, flavonoids and polysaccharides from Gynostemma pentaphyllum stems and leaves. The solvents used for the extraction of the phytochemicals were aqueous ethanol solutions at decreasing concentrations. The optimal ethanol concentration for saponins flavonoids and polysaccharides was 80%, 30% and 5%, respectively, resulting in an extraction efficiency of 8.75%, 0.75% and 3.38%, respectively. The successive extraction procedure showed more efficient utilization of raw materials than single extraction procedures and therefore could provide references for the comprehensive utilization of Gynostemma pentaphyllum.

Key words: Gynostemma pentaphyllum, saponins, flavonoids, polysaccharides, successive extraction

CLC Number:

O629.9

Qing-Hao WANG. Optimization of Successive Extraction of Saponins, Flavonoids and Polysaccharides from Gynostemma pentaphyllum Stems and Leaves[J]. FOOD SCIENCE, 2012, 33(22): 63-66.

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