Abstract:

An HPLC method for the determination of taurine in Ostrea rivularis was established. The optimal conditions of
experimental parameters such as reaction temperature and mobile phase flow rate were established. The HPLC method was
validated with respect to accuracy, precision and limit of quantification. When Ostrea rivularis samples were extracted by a
ultrasonic-assisted extraction method, the resulting extract was separated on a C18 column following pre-column derivatization with
dansyl chloride, and quantitative detection was performed with a UV detector, the developed standard curve of taurine was linear
over the range of 0.2 to 2.2 μg/L, with correlation coefficient (R2) of 0.9932, the limit of detection (RSN = 3) was 1.2 × 10-5 μg/mL,
and the spike recoveries were between 92.24% and 96.73%, with relative standard deviation (RSD) of 6.42%. When the HPLC
method was based on separation on an Amino-Na column followed by post-column derivatization with orthophthalaldehyde
and fluorescence detection, the proposed standard curve of taurine was linear over range between 0.2 μg/mL and 2.2 μg/mL
(R2 = 0.9905), the limit of detection (RSN = 3) was 0.022 μg/mL, and the spike recoveries were between 92.14% and 96.73%,
with RSD of 0.81%. In conclusion, the two methods are simple, rapid, reliable and high accurate and precise and can be used to
determine taurine in Ostrea rivularis from different parts of the South China Sea with no significant differnce.

Key words: Ostrea rivularis, taurine, high performance liquid chromatography (HPLC), derivatization