Isolation and Activity of Antimicrobial Peptide from Ants

doi:10.7506/spkx1002-6630-201309016

Abstract

Abstract:

Objective: To isolate antimicrobial peptide from ants and explore its biological activity. Methods: Wild red ants
from Changbai Mountain were used as the raw material, ethanol-leaching method was used to extract the crude peptide and
heating-column chromatography was used for purification. The relative molecular mass and the bacteriostatic activity of
small molecule polypeptide with antibacterial activity were determined. Results: The antimicrobial peptide obtained from
this method revealed high purity. Relative molecular mass of the antimicrobial peptide was determined to be 3.746 kD. The
antimicrobial peptide from ants had bacteriostatic capacity to Escherichia coli, Staphylococcus aureus, Salmonella enteritidis
and Bacillus subtilis with minimum antibacterial concentrations of 0.325, 0.65, 0.1625 mg/mL and 0.325 mg/mL. Moreover,
the antimicrobial peptide from ants exhibited high thermal stability.

Key words: ant, antimicrobial peptide, isolation, antimicrobial activity

CLC Number:

Q514

SU Xiang，CUI Jing-ai，CHEN Xiao-ping*. Isolation and Activity of Antimicrobial Peptide from Ants[J]. FOOD SCIENCE, 2013, 34(9): 70-73.

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