Adsorption of Bovine Serum Albumin by Reactive Red 120 Modified Magnetic Chitosan Microspheres

doi:10.7506/spkx1002-6630-201321014

Abstract

Abstract:

Magnetic chitosan microspheres were prepared by inverse suspension cross-linking method and then modified
with Reactive Red 120. Adsorption performance of the Reactive Red 120 modified magnetic chitosan microspheres were
assessed using bovine serum albumin (BSA) as the target protein. The impact of pH, ionic strength, adsorption time
and initial concentration of BSA on the amount of BSA absorbed were studied. The results showed that all pH, ionic
strength, adsorption time and initial concentration of BSA had significant impact on the amount of BSA adsorbed. The
maximum adsorption of BSA appeared at pH 5.0 and the adsorption amount decreased with increasing ionic strength. The
adsorption reached an equilibrium in 40 min, and the maximum adsorption capacity of 113 mg/g was obtained when the
initial concentration of BSA was 1 mg/mL. The adsorption behavior was fitted with pseudo second-order kinetic model
and Langmuir thermodynamic model. Using 0.5 mol/L of NaCl at pH 8.0 as the eluent, an elution efficiency of 99.03%
was achieved. The amount of BSA adsorbed by the Reactive Red 120 modified magnetic chitosan microspheres did not
decrease significantly following 5 adsorption-desorption cycles. Our results revealed a promising method for the recovery of
slaughtered animal blood by using the magnetic separation technology.

Key words: magnetic chitosan microspheres, bovine serum albumin, adsorption, reactive red 120

CLC Number:

TS201.25

CAO Miao，LI Zhong-hong*，ZHU Wei，QI Jia，ZHAO Jing-jing. Adsorption of Bovine Serum Albumin by Reactive Red 120 Modified Magnetic Chitosan Microspheres[J]. FOOD SCIENCE, 2013, 34(21): 64-68.

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