Effect of Dissolved Oxygen Controlling Conditions on Production of Extracellular Polysaccharides by Agaricus bisporus MJ-0811

Abstract

Abstract: Agaricus bisporus MJ-0811 was cultured in a 5 L automatic fermenter to explore the effect of dissolved oxygen controlling conditions (stirring speed and aeration) on mycelial biomass, extracellular polysaccharide production, dissolved oxygen concentration, and residual glucose concentration. The results showed that stirring speed and aeration rate had significant impact on the growth of Agaricus bisporus MJ-0811 and the production of extracellular polysaccharides. The highest mycelial biomass (20.81 g/L) and extracellular polysaccharides (3.75 g/L) were obtained when Agaricus bisporus MJ-0811 was cultured for 5 d at 25 ℃ with a stirring speed of 160 r/min and an aeration rate of 0.9 vvm.

Key words: Agaricus bisporus, stirring speed, aeration, dissolved oxygen, extracellular polysaccharides

CLC Number:

TS202.3

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