FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (2): 72-77.

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Aqueous enzymatic extraction of badanmu oil and protein hydrolysate

  

  • Received:2012-06-23 Revised:2012-12-27 Online:2013-01-25 Published:2013-01-15

Abstract: The aqueous enzymatic process of simultaneously preparing oil and protein hydrolysates from BaDanMu was introduced. Badanmu slurry was treated with complex cell-wall polysaccharides degrading enzymes (cellulase:pectinase = 1:2) and Alcalase sequentially, and the optimal conditions for enzymatic hydrolysis were investigated. Through single factor experiment and the orthogonal experiment, the optimum conditions for extraction of badanmu oil are as follows: solid-liquid ratio is 1:5, particle size 30 mesh, cell polysaccharide hydrolysis enzyme content is 3.5%, extraction temperature 40 ℃, extracting time 4 h, alkali extraction pH 9, protease content is 1.5%, 50 ℃ temperature enzyme solution, enzymatic hydrolysis time 2 h;the optimum conditions for extraction of badanmu hydrolyzed protein: solid-liquid ratio is 1:5, particle size 30 mesh, cell polysaccharide hydrolysis enzyme content is 3%, 50 ℃, extraction temperature extracting time 3 h, alkali mention pH 8.5, protease content is 1.5%, 50 ℃ temperature enzyme solution, enzymatic hydrolysis time 2.5 h. Under the optimum conditions, the total extraction rates of free oil and protein hydrolysates are 68.74% and 74.39%, respectively.

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