FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (21): 172-176.doi: 10.7506/spkx1002-6630-201321036

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Optimization of Immobilization of Aspergillus niger Cells with Fructosyltransferase Activity

ZHANG Ying1,YAO Meng-cheng2,JING Wei2,LU Fu-ping2,LI Yu2,*   

  1. 1. Department of Ecology, Environmental Management College of China, Qinhuangdao 066004, China;
    2. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
  • Received:2012-09-06 Revised:2013-09-26 Online:2013-11-15 Published:2013-10-28
  • Contact: LI Yu E-mail:liyu@tust.edu.cn

Abstract:

Aspergillus niger cells with fructosyltransferase were immobilized by entrapping into carrageenan. Five immobilization
parameters were primarily studied by one-factor-at-a-time method, and the optimization of three of them (concentration of carrageenan,
glutaraldehyde and polyethylene polyamine, PEPA) was explored by using a Box-Behnken design coupled with response surface
methodology. The results showed the optimal process conditions were 9.4 g/L carrageenan, 10 g/L glutaraldehyde, 15 g/L KCl, 9 g/L PEPA and
100 g/L Aspergillus niger cells. Under the optimal conditions, the maximum yield of fructooligosaccharides (FOS)
was 40.12%, which was consistent with the predicted value, and the established model was reliable in prediction.

Key words: fructooligosaccharide, Aspergillus niger, fructosyltransferase, immobilized cells, response surface methodology

CLC Number: