FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (5): 213-217.doi: 10.7506/spkx1002-6630-201305044

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Regulation of Exopolysaccharide Produced by Lactobacillus acidophilus NCFM on Immune-Related Genes in Mice

YANG Xiang-yi1，MAN Chao-xin2，LIU Ying1，WANG Jin-yu1，LANG You1，DONG Xin-yue1，YAN Tian-wen1，JIANG Yu-jun1, 2,*   

  1. 1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science and Engineering, Northeast Agricultural University, Harbin 150030, China；2. National Research Center of Dairy Engineering and Technology, Northeast Agricultural University, Harbin 150086, China
  • Received:2012-10-10 Revised:2013-01-19 Online:2013-03-15 Published:2013-04-16
  • Contact: JIANG Yu-jun E-mail:yujun_jiang@163.com

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Abstract: Objective: Exopolysaccharide (EPS) is a metabolic product of probiotics with antitumor, immunomodulatory and antioxidant functions. The aim of this study is to explore the effect of EPS produced by Lactobacillus acidophilus NCFM on immune-related genes in mice. Methods: Viscera coefficients were analyzed through mouse model. Real-time PCR was used to evaluate the expression of immune-related genes in mice after extracting total RNA from intestinal tissues. Results: The results indicated that the viscera coefficients revealed an increase with the extension of time and a significant difference in viscera coefficients between experimental group and control group (P＜0.01). The expression of immune-related genes such as CCL2 and IL18 genes exhibited an initial increase and a final decrease with the extension of time and had the highest level on the 4th day. Similarly, a significant difference in the expression of these genes between experimental group and control group was observed (P＜0.001). Conclusion: EPS can increase the relative weight of immune viscera, and affect the differential expression of immune-related genes. EPS produced by L. acidophilus NCFM can enhance immune function.

Key words: Lactobacillus acidophilus NCFM, exopolysaccharide (EPS), viscera coefficient, immune-related gene

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