Establishment of Determination Method for Flavonoids in Honey and Distribution of Flavonoids in 5 Kinds of Characteristic Honey from Yunan

doi:10.7506/spkx1002-6630-201306032

Abstract

Abstract: A method for the determination of flavonoids in honey by SPE/HPLC was developed. The sample was extracted with acidified water (pH 2) and centrifuged, then enriched and cleaned-up by oasis HLB. The chromatographic separation was conducted on a VP-ODS (150 mm × 4.6 mm, 5 μm) column at the detection wavelength of 270 nm. The elution was completed by the mobile phase composed of methanol and water (pH 3) at the ratio of 60:40 (V/V) at the flow rate of 0.7 mL/min. The detection limits were 0.05 mg/kg for rutin and myricetin, and 0.04 mg/kg for quercetin and kaempferol, respectively. The recovery rates of rutin, myricetin, quercetin and kaempferol were 91%–92%, 82%–85%, 84%–86% and 86%–89%, respectively. Five kinds of characteristic honey from Yunnan were detected to reveal a significant difference in flavonoids. Therefore, flavonoids may be as the markers for the identification of honey.

Key words: flavonoids, characteristic honey, high performance liquid chromatography (HPLC)

CLC Number:

S896.1

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