FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (21): 136-139.doi: 10.7506/spkx1002-6630-201321028

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Isolation and Hydrogen Sulfide Formation of Haploid Strains of Saccharomyces cerevisiae

LIU Mei-ling,LIU Yan-lin   

  1. 1. College of Enology, Northwest A&F University, Yangling 712100, China;
    2. Shaanxi Engineering Research Center for Viti-viniculture, Yangling 712100, China
  • Received:2012-09-19 Revised:2013-09-23 Online:2013-11-15 Published:2013-10-28
  • Contact: Yanlin Liu E-mail:yanlinliu@nwsuaf.edu.cn

Abstract:

In order to study the genetic backgrounds of hydrogen sulfide production in Chinese wild Saccharomyces
cerevisiae, 4 wild yeast spores were randomly separated and isolated using BIGGY agar. The characteristics of hydrogen
sulfide formation in haploids and diploids were studied. Results showed that all the tested wild strains had low sporulation
rate ranging from 19.01% to 43.41%. All the diploid parents showed high capability for H2S production but at varying levels.
Among the 15 haploid strains, 4 were classified as high yield strains, 5 as middle yield strains and 6 as low yield strains. The
screened haploid strains were important materials for further study of genetic background and metabolic mechanism of H2S
production in Saccharomyces cerevisiae. The results of our study are expected to provide useful references for exploration of
future genetic resource and cultivation of new strains.

Key words: Saccharomyces cerevisiae, random spores separation, haploid, hydrogen sulfide

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