Effect of RS4-type Resistant Starch on Intestinal Villus Morphology and Small Intestinal Flora in High-fat Diet-fed C57BL/6J Mice

doi:10.7506/spkx1002-6630-201321067

Abstract

Abstract:

This study aimed to examine the effect of cross-linked octenyl succinic anhydride modified sweet potato starch
(CLOSA-SPS), citric acid acetylation modified sweet potato starch (CAAC-SPS) and hydroxypropyl cross-linked modified
sweet potato starch (HPCL-SPS) as RS4-type resistant starch (RS) on the small intestinal microenvironment of C57BL/6J
mice fed high-fat diet. A total of 72 male C57BL/6J mice were randomly divided into six groups: normal control group (CL, fed
basal diet), high-fat group (HF, given high-fat diet), high-fat diet containing sweet potato starch group (HF-SPS,150 g/kg), highfat
diet containing CLOSA-SPS,CAAC-SPS or HPCL-SPS groups (HF-CLOSA-SPS, HF-CAAC-SPS and HF-HPCL-SPS,
respectively 150 g/kg). The mice were sacrificed after 12 weeks to determine the intestinal villus height and crypt depth and
to explore the effect of the RS4-type RS on the intestinal flora of C57BL/6J mice fed high-fed diet. Results showed that HF
and HF-SPS diets significantly reduced the intestinal villus height and increased crypt depth in mice, which resulted in a
decreased ratio of villus height and crypt depth (V/C), compared to the control group. The RS4-type resistant starches were
able to increase the intestinal villus height, reduce the crypt depth and thus increase V/C ratio. The effect of the HF-CAACSPS
and HF-HPCL-SPS were higher than that of the HF-CLOSA-SPS, which were significantly different (P ＜ 0.05) from
the HF and HF-SPS. High-fat diet changed the intestinal flora of mice. The UPGMA analysis showed that the HF group and CL
group were significantly different and that the diversity index of the former was significantly lower than that of the latter (P ＜ 0.05).
The intestinal flora of mice fed the RS4-type RSs was quite similar to that of the CL group and the diversity indices were
significantly greater than those of the HF group and HF-SPS group (P ＜ 0.05). In conclusion, CAAC-SPS, HPCL-SPS and
CLOSA-SPS, the 3 kinds of RS4-type RS could improve the morphology of intestinal villus and intestinal flora of C57BL/6J
mice fed high-fat diet.

Key words: RS4-type resistant starch, C57BL/6J mice, intestinal villus morphology, denaturing gradient gel electrophoresis, intestinal flora

CLC Number:

TS23

WU Ying-long，WANG Wen-ting. Effect of RS4-type Resistant Starch on Intestinal Villus Morphology and Small Intestinal Flora in High-fat Diet-fed C57BL/6J Mice[J]. FOOD SCIENCE, 2013, 34(21): 333-338.

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