FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (7): 284-288.doi: 10.7506/spkx1002-6630-201307060

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Effect of Polysaccharides from Lycium barbarum on Cisplatin-Induced Apoptosis of Human Embryonic Kidney Cells

GUO Pei-pei,GAO Li-ping*,LENG Hong-tao,GUO Zhuo-yu,LU Zheng   

  1. Beijing Municipal Key Laboratory of Biologically Active Substances and Functional Food, College of Arts and Sciences, Beijing Union University, Beijing 100191, China
  • Received:2012-04-25 Revised:2013-02-21 Online:2013-04-15 Published:2013-03-20
  • Contact: GAO Li-ping E-mail:gaoliping@ygi.edu.cn

Abstract: This study aimed to explore the effect of polysaccharides from Lycium barbarum (LBP) on the cisplatin-induced apoptosis of human embryonic kidney cells and to investigate its possible mechanism. MTT method was used to observe the effect of LBP and cisplatin on the cell viability of HEK293 cells. CDDP-induced apoptosis was analyzed by flow cytometry and Western blotting was applied to determine the expression of apoptotic gene Bax and anti-apoptosis gene Bcl-2. LBP was able to antagonize cisplatin-induced cytotoxicity in a concentration-dependent manner. Flow cytometry revealed that increasing LBP concentrations led to a decrease in the apoptosis rate and pro-apoptotic Bax gene expression while antiapoptotic gene Bcl-2 expression was enhanced. LBP demonstrated a preventive effect on cisplatin-induced injury in EK293 cells. The mechanism may be related to its capacity of enhancing anti-apoptotic gene Bcl-2 expression and inhibiting proapoptotic gene Bax expression.

Key words: cisplatin, LBP, HEK293, apoptosis

CLC Number: