Optimization of Fermentation Conditions for Exopolysaccharide Production by Bacillus amyloliquefaciens LPL061

doi:10.7506/spkx1002-6630-201307039

Abstract

Abstract: In order to improve expolysaccharide (EPS) production by Bacillus amyloliquefaciens LPL061, the medium and fermentation conditions were optimized by one-factor-at-a-time design and response surface analysis. The optimal culture medium consisted of 22 g/L sucrose and 18.4 g/L yeast extract at pH 7.0. The optimal fermentation conditions were 28 ℃, 220 r/min shaking speed, 3% inoculum size and 24 h. Under these conditions, the EPS yield was 4.46 g/L, 1.51-fold higher than before the optimization.

Key words: Bacillus amyloliquefaciens, exopolysaccharide, fermentation condition optimization, response surface analysis

CLC Number:

TS201.3

LI Yan-yan，ZHANG Cai，FAN Yi，LIU Li-sha*，LI Ping-lan，HAN Yu-zhu. Optimization of Fermentation Conditions for Exopolysaccharide Production by Bacillus amyloliquefaciens LPL061[J]. FOOD SCIENCE, 2013, 34(7): 185-189.

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