FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (21): 283-291.doi: 10.7506/spkx1002-6630-201321057

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Lycopene Biosynthesis in Rhodopseudomonas palustris

ZHANG Tong，ZHU Rui-yan，HUI Bo-di，YANG Bo-yuan   

  1. 1. College of Applied Arts and Science, Beijing Union University, Beijing 100191, China；
    2. Hebei New Bio-energy Engineering Research Center, Qinhuangdao 066004, China
  • Received:2012-10-09 Revised:2013-10-11 Online:2013-11-15 Published:2013-10-28
  • Contact: Bodi HUI E-mail:bodi_hui@buu.edu.cn

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Abstract:

This research aims to study the biosynthesis of lycopene in Rhodopseudomonas palustris and explore the possibility
for Rhodopseudomonas palustris to be a novel source of natural lycopene. The metabolic products of R. palustris extracted with
tetrahydrofuran (THF) were analyzed by HPLC and HPLC-MS. The results showed that the main carotenoids in R. palustris were
rhodopin, 3,4-dehydrorhodopin and lycopene. R. palustris was cultivated in SMN supplemented with carbon source. Massive
lycopene accumulation was observed during a period after the beginning of the logarithmic phase, followed by a decline at the
stable phase. The optimal carbon source, nitrogen source and pH were determined. Under the optimal cultivation conditions, the
yield of lycopene reached a maximum level of 0.2456 μg/mL, and the relative yield was 0.1682 μg/g.

Key words: lycopene, Rhodopseudomonas palustris, biosynthesis, culture optimization