Isolation and Identification of Spoilage Microorganisms from Sichuan Pickles

doi:10.7506/spkx1002-6630-201321042

Abstract

Abstract:

Eight spoilage microbes with different colony morphologies were isolated from four different spoiled
Sichuan pickle samples by using PDA and NA media. Unspoiled samples were spoiled when artificially inoculated
with these strains. They were identified as 4 bacteria including 3 B. subtilis and 1 B. altitudinis and 4 yeasts including
2 P. manshurica, 1 P. kudriavzevii and 1 C. tropicalis through PCR amplification and sequence analysis of 16S
rDNA/18S rDNA and phylogenetic tree analysis.

Key words: Sichuan pickles, spoilage microorganisms, isolation, identification

CLC Number:

TS201.3

AO Xiao-lin1，CAI Yi-min2，XIA Jiao1，ZHAO Ke3，PU Biao1，ZHANG Xiao-ping3. Isolation and Identification of Spoilage Microorganisms from Sichuan Pickles[J]. FOOD SCIENCE, 2013, 34(21): 204-208.

References

[1] Maki，M. (2004). Lactic acid bacteria in vegetable fermentations. In Salminen, S., Wright, A. V., & Ouwehand, A. Lactic acid bacteia: microbiological and functional aspects (PP. 419 - 430). New York: Marcel Dekker.
[2] 刘小艺, 阚建全, 阮志勇. 应用响应面优化泡菜用微生物的富集培养基[J]. 食品工业科技, 2011, 32(5): 183-189.
[3] 阳辛凤, 高秋芳, 李敏锡等. 菌落PCR快速扩增工业酿酒酵母基因组DNA片段[J]. 生物技术通报,2010,(9): 215-219 .
[4] JAMES B R, JACK H. PCR primers that amplify fungal rRNA genes from environmental samples[J]. Apply Environment. Microbiology, 2000, 66(10): 4356-4360.
[5] 武香玉, 徐海燕, 辛国芹等. 一株乳酸菌的分离鉴定及生物学特性研究[J]. 畜牧与饲料科学, 2012, 33(3): 1-3.
[6] 敖晓琳, 张小平, 史令等. 四川泡菜中两株优良乳酸菌的鉴定及不同发酵条件对其发酵泡菜品质的影响[J]. 食品科学, 2011, 32( 11): 152-156.
[7] 李洁, 孙姝, 朱科学等. 半干面腐败菌的分离与鉴定[J]. 食品科学, 2012, 33(5): 183-187.
[8] 闫国宏, 傅力, 肖春芳等. 新疆番茄酱中枯草芽孢杆菌耐热性的研究[J]. 食品研究与开发, 2008, 29(11): 88-90.
[9] 胡志明,谢广发, 吴春等. 黄酒大罐发酵醪液中原核微生物的初步研究[J]. 酿酒科技, 2009, 182(8): 58-61.
[10] 毛云莹. 成都平原农田土壤芽孢杆菌[D]. 雅安: 四川农业大学, 2011.
[11] 中国科学院微生物研究所.常见和常用真菌[M]. 北京: 中国科学出版社, 1973: 80-90.
[12] 章银珠, 姚卫蓉. 变质传统发酵陈化黄酒微生物的分离和鉴定[J]. 中国酿造, 2008, 198(21): 62-66.
[13] SAEZ J S, LOPES Ch A, KIRS V E, et al. Production of volatile phenols by Pichia manshurica and Pichia membranifaciens isolated from spoiled wines and cellar environment in patagonia[J]. Food Microbiology, 2011, 28(3): 503-509.
[14] AKABANDA F, OWUSU K, JAMES T D,et al. Taxonomic and molecular characterization of lactic acid bacteria and yeasts in nunu, a Ghanaian fermented milk product[J]. Food Microbiology, 2012,1-7.
[15] DHALIWAL S S, OBEROI H S, SANDHU S K, et al. Enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of Pichia kudriavzevii[J]. Bioresource Technology, 2011, 102(10): 5968-5975.
[16] GOLOMB B L , MORALES V J, ALESIA Y, et al. Effects of pectinolytic yeast on the microbial composition and spoilage of olive fermentations[J]. Food Microbiology, 2013, 33(1): 97-106.
[17] 侯风伶, 申志新, 张淑红. 热带假丝酵母菌引起保健饮品变质的检测[J]. 中国卫生检验杂志, 2002,(06):709.