Apoptosis induction on rat vascular smooth muscle cells of Laminaria japonica polysaccharide

Abstract

Abstract:

Purpose: To study the mechanism of apoptosis induced by Laminaria japonica polysaccharide. Methods: The proliferation of vascular smooth muscle cells induced by basic fibroblast growth factor (bFGF) was evaluated using MTT assay; The morphological changes of vascular smooth muscle cells were investigated by fluorescence microscope; The cell cycle distribution of vascular smooth muscle cells was determined using flow cytometry; The apoptotic factors were evaluated by Western-blot assay. Results: Laminaria japonica polysaccharide exhibited anti-proliferative activity on vascular smooth muscle cells in a dose-dependent manner. Further investigation indicated that Laminaria japonica polysaccharide induced vascular smooth muscle cells apoptosis. The apoptotic mechanism was related to the induction of S phase arrest, as well as regulating Bcl-2 family proteins expressions. Conclusions: Laminaria japonica polysaccharide could induce apoptosis.

CLC Number:

Q539

PENG Zhen-fei1,2，FANG Zhe-xiang1，LIU Min1，CHEN Li1，ZHANG Qi-qing1,*. Apoptosis induction on rat vascular smooth muscle cells of Laminaria japonica polysaccharide[J]. FOOD SCIENCE, 2013, 34(21): 344-348.

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