Abstract:

Isomaltooligosacharide, fructooligosaccharides, fucosylated oligosaccharide and sialyloligosaccharide have
been widely applied in milk powder for infants and young children because of its special physiological effect to promote the
growth of bifidobacterium. These functional oligosaccharides are produced by α-glycosyltransferase, fructosyltransferase,
fucosyltransferase and sialyltransferase, using glucose, sucrose and lactose as substrates, respectively. This paper
reviews recent research advances in the microbial souces, properties, activity and expression of these enzymes and their
applications in the synthesis of functional oligosaccharides in order to provide refereces for the development of highly active
glycosyltransferases and the improvement of their applicability for the production of functional oligosaccharides.

Key words: &alpha, -glycosyltransferase；fructosyltransferase；fucosyltransferase；sialyltransferase