FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (9): 1-4.doi: 10.7506/spkx1002-6630-201309001

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Structural Identification of Seleno-peptides and Seleno-amino Acids in Se-enriched Corn Protein Hydrolysates

WANG Zhen-zhen,ZHANG Jiu-liang,WANG Chi,HOU Tao,ZHANG Yan,HE Hui*   

  1. Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology,
    Huazhong Agricultural University, Wuhan 430070, China
  • Received:2013-03-20 Revised:2013-04-11 Online:2013-05-15 Published:2013-05-07
  • Contact: HE Hui E-mail:hehui@mail.hzau.edu.cn

Abstract:

In order to clarify the combination form of selenium in corn proteins, the structures of seleno-peptides in Se-enriched
corn protein hydrolysates were identified by HPLC-MS/MS method coupled with mass spectrum database and seleno-amino
acids were identified by HPLC-ICP-MS method. Five seleno-peptides were confirmed as SeMet-MeSeCys-Glu, Met-MeSeCys-
Glu, MeSeCys-Glu-Asp, Ile-MeSeCys-Glu and γ-Glu-MeSeCys, and four seleno-amino acids were identified as selenomethionine
(SeMet), eleno-Ethionine (SeEt), Se-methylseleno-L-Cysteine (MeSeCys) and selenocystine (SeCys2), respectively. Seleno-amino
acids and dipeptide were been quantified using standard samples. Selenomethionine (SeMet) revealed the highest content (586.3 ±
49.5) ng/g, and Selenocystine (SeCys2) revealed the low content (102.6 ± 9.0) ng/g. Based on the above results, all five selenopeptides
contain MeSeCys, and only one contains SeMet. The major seleno-amino acid was SeMet.

Key words: Se-enriched corn, seleno-peptides, seleno-amino acids, HPLC-MS/MS, HPLC-ICP/MS

CLC Number: