Optimization of solid-state fermentation medium of α-galactosidase produced by Aspergillus niger ZJUQH with response surface methodology

doi:10.7506/spkx1002-6630-201309044

Abstract

Abstract:

In order to enhance the production of α-galactosidase by Aspergillus niger ZJUQH, response surface
methodology was applied to optimize the solid culture medium. A fractional factorial design was used to investigate the
main factors affecting α-galactosidase yield. The results showed that two factors played important roles in the production of
α-galactosidase. Central composite design and response surface analysis were applied to establish a second-order regression
equation model for the yield of α-galactosidase. At last, the optimal fementation condition were obtained by response surface
analysis as follows: peptone 0.6 g, Na2HPO4·12H2O 0.08 g, ratio of solid to water 0.41 and inoculum quantity 1.5 mL/5g . Under
the optimal conditions, the activity of α-galactosidase reached (77.21±2.01) U/g, which was increase by 57.41% compared
with (49.05 ± 2.11) U/g before the oprimization, that is, peptone 0.4 g, Na2HPO4·12H2O 0.08 g, ratio of solid to water 0.33
and inoculation quantity 1.5 mL/5 g.

Key words: response surface methodology；Aspergillus niger ZJUQH；&alpha, -galactosidase；solid-state fermentation

CLC Number:

Q939.97

XU Teng-yang，FANG Ruo-si，DONG Ya-chen，CHEN Qi-he*. Optimization of solid-state fermentation medium of α-galactosidase produced by Aspergillus niger ZJUQH with response surface methodology[J]. FOOD SCIENCE, 2013, 34(9): 216-219.

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