Abstract:

DNA extraction is the difficulty in detecting genetically modified (GM) foods. DNA extraction with highmagnetic
response microspheres coupled with qRT-PCR has been applied to establish a rapid new method for the detection
of transgenic components in foods. The extraction efficiency of geomic DNA from deeply processed products of potato
and soybean with high-magnetic response microspheres was investigated in this study. The DNA samples obtained were
analyzed by PCR and qRT-PCR and the results were compared with those obtained with a commerical kit. Complete,
high pure, amplifiable DNA templates which were suitable for qRT-PCR were obtained by the proposed method and even
its extraction efficiency was better than that of the commercial kit. Therefore, this method is particularly applicable for
extracting DNA fragments from deeply processed products of potato and soybean.

Key words: magnetic microsphere, DNA extraction, fluorescence quantitative polymerase chain reaction, genetically modified food detection, deeply-processed food