Abstract:

Flavonoids from Zanthoxylum bungeanum were separated by high-speed countercurrent chromatography. The separation was performed using a two-phase solvent system composed of chloroform, methanol, n-butanol and water(4:3:0.5:2, V/V) at a flow rate of 2.0 mL/min, and rotation speed and detection wavelength were set at 850 r/min and 254 nm, respectively. Six flavonoids were detected by HPLC. The purities of peaks 1, 2, 3, 4, 5 and 6 were 72.7%, 67.8%, 89.6%,96.5%, 94.1% and 91.3%, respectively. Their structures were characterized as foeniculin, quercetin-7-glucoside, rutin and quercetin-3-galactoside by MS and 1H-NMR.

Key words: high-speed countercurrent chromatography (HSCCC), flavonoids, Zanthoxylum bungeanum, separation