Abstract:

Based on static and dynamic adsorption performance, DA201-C type macroporous resin was selected as a more
appropriate carrier to separate and purify ACE inhibitory peptides from the pepsin hydrolysate of porcine hemoglobin. The
optimal process parameters for dynamic adsorption and desorption were determined as follows: 60 mL of 40 mg/mL samples
at pH 5.0 and 0.5 mL/min of loading flow rate, followed by desorption with 75% ethanol at a flow rate of 1.0 mL/min.
The IC50 of the ACE inhibitory peptides obtained under the optimized conditions was 0.87 mg/mL. Comparison of static
adsorption and desorption with dynamic adsorption and desorption indicated that the static adsorption rate of ACE inhibitory
peptides on DA201-C was 59.4%, which was higher than the dynamci adsorption rate of 48.1%.

Key words: macroporous resin, adsorption, porcine hemoglobin, DA201-C, ACE inhibitory peptides