Abstract:

The purificaiton of total flavonoids from hop (Humulus lupulus L.) was investigated. Colorimetric determination
of total flavonoids was carried out with NaNO2-Al(NO3)3-NaOH. We compared the efficiencies of nine types of macroporous
adsorption resin for static adsorption and desorption of hop flavonoids. In addition, the effects of pH, concentration and
flow rate of sample and pH, concentration and dose of eluent on the purificaiton efficiency of flavonoids were examined.
Polyamide was identified as the most appropriate resin for purifying total flavonoids from hop. The optimal purification
conditions were obtained when 10 mL of sample was loaded onto an 1-g polyamide column at a flow rate of 1.0 mL/min
followed by desorption with 8 BV of 80% methanol. Under these conditions, the yield of flavonoids from hop was 32.69%
with a purity of 50.05%.

Key words: Humulus lupulus L., total flavonoids, purification, polyamide