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Preparation of Time-Temperature Indicator Reaction System Based on Glucoamylase

ZHENG Guang-lin,QIAN Jing*,FENG Qin   

  1. School of Mechanical Engineering, Jiangnan University, Wuxi 214122, China
  • Online:2013-06-25 Published:2013-06-17

Abstract:

Color changes occuring during the course of reaction between glucoamylase and starch in the presence of iodine
solution as an indicator display the cumulative effects of time and temperature. A time-temperature indicator reaction
system was developed with 10 mL of 40 g/L maltodextrin as reaction substrate, 3 mL of 1 g/L iodine solution and variable
amounts of glucoamylase. The time-temperature indicator was widely applicable for products at different times of cold chain
transportation. The reaction system with 50 μL of glucoamylase was suitable for low temperature controlled packaging for
3 to 4 d at about 4 ℃, and the activation energy was found to be 75.94 kJ/mol. This system had potential application for
chilled fresh meat, which is vulnerable to quality deterioration caused by lipid peroxidation.

Key words: time-temperature indicator, glucoamylase, cold chain transportation, activation energy, lipid oxidation