Uncertainty Evaluation for the Determination of Cholecalciferol in Infant Formula Milk Powder by Ultra Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201312030

Abstract

Abstract:

Based on JJF 1059—1999 Evaluation of the Uncertainty of Determination and Expression, a mathematical model
was established to assess the uncertainty of analyzing cholecalciferol (VD3) in infant milk powder by ultra performance
liquid chromatography (UPLC). Factors contributing to the uncertainty were analyzed, quantified and synthesized. The
expanded uncertainty of VD3 determination for 10.0 g of samples was 0.2091 μg. Our results showed that the uncertainty
of measurement was mainly caused by the preparation of standard solution, the establishment of the standard curve and the
uncertainty of stochastic factors during the determination of samples. The evaluation method is reasonable, clear, simple,
accurate, and suitable for the evaluation of uncertainty in UPLC determination.

Key words: ultra-performance liquid chromatography, infant formula milk powder, cholecalciferol, uncertainty evaluation

FAN Yao，HUANG Cui-li，WANG Li-qing，REN Guo-pu，ZHOU Gui-ping，CHEN Hong-tao. Uncertainty Evaluation for the Determination of Cholecalciferol in Infant Formula Milk Powder by Ultra Performance Liquid Chromatography[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201312030.

[1]	ZHAI Hongwen, FAN Sufang, WANG Juan, CAO Meirong, MA Junmei, REN Xiaowei, LI Qiang, ZHANG Yan. Recent Progress in Measurement Uncertainty and Its Application in Food Inspection and Detection [J]. FOOD SCIENCE, 2021, 42(5): 314-320.
[2]	ZHANG Jiachan, WANG Changtao, ZHAO Dan, WANG Chengtao, SUN Baoguo. Qualitative and Quantitative Analysis of Sea Buckthorn Seed Extract and Its Antioxidant Effect in Liver of Aging Mice [J]. FOOD SCIENCE, 2019, 40(2): 229-238.
[3]	CHEN Xinxin, LU Jing, LIU Lu, PANG Xiaoyang, ZHANG Shuwen, LI Cheng, Lü Jiaping. Determination of 12 Oligosaccharides in Human Milk by Ultra-Performance Liquid Chromatography-Mass Spectrometry [J]. FOOD SCIENCE, 2018, 39(4): 138-143.
[4]	SUN Xiaodong, HAO Jie, MAO Ting, SHAO Ruiting, JIANG Jie. Determination of 14 Mycotoxins in Liquid Milk by Solid-Phase Extraction Coupled with Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2018, 39(18): 292-301.
[5]	LI Xiaolei, CHEN Jun, YANG Chaofen, YANG Jun. Determination of Six Antilipemic Chemicals Illegally Added in Antilipemic Functional Foods by UPLC-MS/MS [J]. FOOD SCIENCE, 2018, 39(18): 320-325.
[6]	CHANG Qiang, SU Minghua, CHEN Qingxi, ZENG Biyu, LI Huihua, WANG Wei. Determination of Polyphenols and Antioxidant Activity in Chinese Olive Fruits by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2017, 38(24): 150-158.
[7]	ZHU Zhenbao, ZHANG Yali, JIA Wei, KONG Xianghong, HE Qiang. Determination of 7 Artificial Dyes in Plant Extracts by Ultra-Performance Liquid Chromatography-Quadrupole/Electrostatic Field Orbit Trap High-Resolution Mass Spectrometry [J]. FOOD SCIENCE, 2017, 38(24): 196-201.
[8]	BO Haibo, YAO Zhimin, XING Yuxiu, YAO Lihong, JI Shengjun, WANG Jingying. Simultaneous Determination of Multiple Kinds of Plastic Additives in Highland Barley Liquor from Qinghai by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2017, 38(12): 298-303.
[9]	CHEN Meijun1, WANG Min1, KANG Meijuan1, DUAN Qihui2, SHANG Jiaowen2, SHAN Yi1,*. Simultaneous Determination of 11 B Vitamins in Infant Formula by UPLC-MS/MS [J]. FOOD SCIENCE, 2016, 37(4): 144-153.
[10]	LUO Yiyuan, LIU Juanxiu, LIU Xunhong, LAN Caiwu, XU Li, HOU Ya, MA Yang. Analysis of Nucleosides and Nucleobases of Polygonum multiflorum by Stepwise Regression Analysis [J]. FOOD SCIENCE, 2016, 37(2): 104-108.
[11]	ZHOU Xiumei, LEI Baoliang, ZHANG Fengping, LIU Yaomin, FAN Xiuli. Uncertainty Evaluation for the Determination of Starch Content in Manihot esculenta by Polarimetric Method [J]. FOOD SCIENCE, 2016, 37(12): 144-147.
[12]	HE Xiao-min1,2, CHEN Hao3,*. Determination of Eight Perfluorinated Compounds in Fish Muscles by Isotope Dilution Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2014, 35(8): 193-197.
[13]	ZHU Yan-mei1,2, ZHAO Qi-yang1, CHEN Wei-jun1, JIAO Bi-ning1,2,3,*, ZHANG Yao-hai1. Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry Combined with QuEChERS for the Quick Detection of Fenbutatin Oxide Residue in Citrus [J]. FOOD SCIENCE, 2014, 35(4): 111-115.
[14]	ZHANG Jin-yan, HU Li-fang, LI Wei-hong, GUO Xiao-pei, LUO Lin-guang*. Uncertainty Evaluation for the Determination of Basic Orange in Chili Powder by High Performance Liquid Chromatography [J]. FOOD SCIENCE, 2014, 35(20): 213-216.
[15]	HUO Xiao-min. Uncertainty Evaluation for Determination of HCH and DDT in Dried Cucumber by Gas Chromatography [J]. FOOD SCIENCE, 2013, 34(8): 244-248.

Uncertainty Evaluation for the Determination of Cholecalciferol in Infant Formula Milk Powder by Ultra Performance Liquid Chromatography

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments