Abstract:

A novel fluorescence probe 2-APC was synthesized based on nucleophilic substitution reaction of 2-aminopyridine
and 2-pyridinecarbaldehyde in this study. The reaction product was characterization by melting test, elemental analysis,
infrared spectrum and 1HNMR. A fluorescence probe method for superoxide anion radical detection was established using
the probe. The optimum reaction conditions were pH 8.2, 40 ℃ and 40 min. The conditions of fluorimetric determination
were established as follows: λex = 295 nm, λex = 365 nm and slit width = 5 nm. In the range from 0.4 × 10-6 to 8.0×10-6 mol/L,
relative fluorescence intensity (y) and pyrogallol concentration (x) showed a good linear relationship: y =37.567x + 55.581,
R2 = 0.9834. Superoxide anion radical (O2 － •) scavenging activity of L-ascorbic acid was evaluated using the method and an
IC50 of 0.182 mmol/L was obtained.

Key words: superoxide anion radical, fluorescence probe, synthesis, application