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Screening and Identification of Antioxidants in Glycyrrhiza Extract Rich in Glycyrrhizine by High Performance Liquid Chromatography Coupled with Post Column DPPH Assay

XING Zhen-zhen,MENG Qing-yan,LU Ya-ling,ZHANG Hong-xi,SHEN Xue-hua,LIU Wen-jie   

  1. 1. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Groups, Tarim University, Alar 843300, China;2. College of Life Sciences, Tarim University, Alar 843300, China
  • Online:2013-06-25 Published:2013-06-17

Abstract:

A new method based on HPLC online scavenging DPPH radical activity and HPLC-MS/MS method for
the identification of antioxidants from glycyrrhiza extract rich in glycyrrhizine was developed. The glycyrrhiza extract
was separated by HPLC and the outlet of HPLC photo diode array detector flow cell was connected to a DPPH flow.
Antioxidants were reacted with DPPH in post-column reaction coil and monitored by UV detector at 517 nm. The results
showed that eight compounds with scavenging radical activity were found in the glycyrrhiza extract, which were identified
as formononetin, choerospondin, apioliquiritin, liquiritigenin, liquiritin, echinatin, glyyunnansapogenin F, glabrone and
licochalcone C by MS/MS and UV spectrometry.

Key words: glycyrrhiza extract, high performance liquid chromatography, screening, antioxidants