FOOD SCIENCE

Previous Articles     Next Articles

Development of a High-Performance Liquid Chromatographic Method for Detection of Chlorfluazuron Residues in Fruits Cultivated in Southeast Asian

LÜ Ping,DU Guo-dong,WEI Li-jun,NONG Yao-jing   

  1. 1. Guangxi Zhuang Autonomous Region Research Institute for Subtropical Crops, Nanning 530001, China;
    2. Quality Supervision and Testing Center of Subtropical Fruit and Vegetable, Ministry of Agriculture, Nanning 530001, China
  • Online:2013-06-25 Published:2013-06-17

Abstract:

A analytical method was established to determine chlorfluazuron residues in banana, mango and litchi
cultivated in Southeast Asia by high performance liquid chromatography (HPLC). Samples were extracted acetone
and the extact was then cleaned up on a Florisil column prior to analysis by HPLC with UV detection. The analytical
column was an Agilent Eclip se XDB C18 column (4.6 mm × 250 mm, 5 μm) and the mobile phase was methyl alcoholwater
(90:10, by volume) at a flow rate of 1.0 mL/min. The detection wavelength was set at 258 nm. Quantification was
performed by the external standard method. The results showed that the linear range for chlorfluazuron was in the range
of 0.05-5.00 mg/L with a correlation coefficient of 0.99996. The average recoveries of chlorfluazuron were between 84.7%
and 102%, with a variation coefficient ranging from 2.0% to 6.7%. The limit of quantification of this method was 0.02 mg/kg.
This method met the national requirements for pesticide determination and was applicable to food quality supervision and
control on chlorfluazuron.

Key words: chlorfluazuron, high performance liquid chromatography, fruits