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Frabrication of Benzoyl Peroxide Biosensor Based on Carbon Nanotube-Modified Electrode

DING Jian-ying,PAN Huan-huan,ZHANG Gen-hua,QUAN Ying,ZHAN Yue-hua   

  1. College of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Online:2013-06-25 Published:2013-06-17

Abstract:

An electrochemical enzyme biosensor was developed to determine benzoyl peroxide (BPO) in flour. Carbon
nanotube was modified on the surface of glassy carbon electrode as an electron mediator, followed by coating the electrode
surface with horseradish peroxidase embedded in chitosan sol-gel. A new type of benzoyl peroxide biosensor was fabricated
based on changes in the electrode current response caused by the catalytic action of horseradish peroxidase on BPO. The results
showed that the response current of the sensor was linearly related to BPO concentration over the range of 8.3 × 10-6 mol/L
to 3.4 × 10-3 mol/L with a detection limit of 2.3 × 10-6 mol/L. This biosensor is sensitive, fast and convenient and has the
potential to be applied for the determination of benzoyl peroxide in flour.

Key words: benzoyl peroxide, electrochemical enzyme sensor, carbon nanotube, glassy carbon electrode