Determination of Flavonoids in Fuzhuan Tea by HPLC

doi:10.7506/spkx1002-6630-201312057

Abstract

Abstract:

An HPLC method for the determination of flavonoids in Fuzhuan tea was presented. Chromatographic separation
was performed on Welchrom C18 (250 mm × 4.6 mm, 5 μm) column by graditent elution with methanol as mobile phase A and
0.5% acetic acid as mobile phase B at a flow rate of 0.8 mL/min. Detection wavelength was 295 nm. It took 80 min to complete
the analytical process. The established method was precise, stable and repeatable, with an average recovery of 94.88%. Thanks
to its ease of use, specificity and accuracy, this method can be used for the determination of flavonoids of Fuzhuan tea.

Key words: Fuzhuan tea, high performance liquid chromatography (HPLC), flavonoid

LI Shi，LU Qiang，LI Yin-hua，HUANG Jian-an，LIU Zhong-hua. Determination of Flavonoids in Fuzhuan Tea by HPLC[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201312057.

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Determination of Flavonoids in Fuzhuan Tea by HPLC

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