Rapid Detection of Cronobacter sakazakii Using a Loop-Mediated Isothermal Amplification (LAMP) Assay

doi:10.7506/spkx1002-6630-201312061

Abstract

Abstract:

This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for rapid detection of
Cronobacter sakazakii. The gene sequences of ompA were aligned using the biologic software and four specific primers used for
LAMP were designed and the reaction condition was optimized. DNA templates from different cell concentrations were extracted by
boiling, FTA card and kit methods and detected by the LAMP method to obtain detection limits of 104, 103 CFU/mL and 103 CFU/mL,
respectively. Seven of 12 Cronobacter sakazakii isolates investigated were positive while all the others were negative. This method
may provide a more simple and rapid approach to detecting Cronobacter sakazakii in comparison with PCR method.

Key words: Cronobacter sakazakii, LAMP, detection

REN Li-song，CHEN Meng-xin，CHEN Zhuo，MA Long. Rapid Detection of Cronobacter sakazakii Using a Loop-Mediated Isothermal Amplification (LAMP) Assay[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201312061.

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Rapid Detection of Cronobacter sakazakii Using a Loop-Mediated Isothermal Amplification (LAMP) Assay

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