FOOD SCIENCE

Previous Articles     Next Articles

Development of Double-Antibody Sandwich ELISA for the Detection of the Almond Allergen Amandin

ZHANG Jie-qiong，GAO Shu-xia，SHENG Wei，ZHANG Yan，WANG Shuo   

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology,Tianjin University of Science and Technology, Tianjin 300457, China
  • Online:2013-08-25 Published:2013-09-03

RichHTML

0

PDF (PC)

215

Abstract:

In the present study, allergen amandin from Chinese sweet almond was prepared, and specific antibodies
against the amandin were produced. A double-antibody sandwich enzyme linked immunosorbent assay (ELISA) based
on rabbit polyclonal antibody and mouse polyclonal antibody was then established. The newly established ELISA could
detect amandin at levels as low as (6.36 ± 1.02) μg/L, showing no cross-reactivity with 14 common plant proteins. The
limit of detection for amandin was (12.11 ± 1.70) μg/L in Chinese bitter apricot kernel and (18.95 ± 1.52) μg/L in
almond (Amygdalus communis L.). The sandwich ELISA were used to detect the amandin in spiked samples of Frenchstyle
bread, biscuit and skim milk with recoveries ranging from 78.94% to 125.15%, and relative standard deviation (n = 3)
below 5.81%.

Key words: apricot kernel, amandin, double-antibody sandwich enzyme linked immunosorbent assay (sandwich ELISA)