Abstract:

Molecularly imprinted microspheres (MIMs) were synthesized using florfenicol as template molecule,
methacrylic acid as functional monomer, ethylene glycoldimethacrylate as crosslinking agent, 2,2-azobisisobutyronitrile
as initiator by emulsion-suspension polymerization. An analytical method was established for the determination of
amphenicols residue milk using on-line solid-phase extraction with the microspheres coupled with high performance
liquid chromatography. Some extraction and detection conditions were investigated. Under the optimal conditions, the best
separation and cleanup were obtained. The average recoveries for spiked milk were in the range of 80.6%–96.7% and the
detection limits for thiamphenicol, florfenicol and chloramphenicol were 6.8, 10.9 μg/kg and 27.8 μg/kg, respectively.

Key words: molecularly imprinted, polymers microspheres, on-line solid-phase extraction, amphenicols, milk