FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (21): 24-29.doi: 10.7506/spkx1002-6630-201321006

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in vitro Digestion and Absorption of Polysaccharide from Cyclocarya paliurus Leaves

MIN Fang-fang,NIE Shao-ping,WAN Yu-jun,XIE Ming-yong*   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2013-06-14 Revised:2013-10-08 Online:2013-11-15 Published:2013-10-28
  • Contact: XIE Ming-yong E-mail:myxie@ncu.edu.cn

Abstract:

Polysaccharides were isolated from Cyclocarya paliurus leaves by water extraction and ethanol precipitation and
their physicochemical properties were determined. The digestion and absorption of the polysaccharides were studied in vitro by
employing a simulated upper gastrointestinal model and analyzing molecular weight, reducing sugar and free monosaccharide
contents of digested products at different digestion times. The results indicated that the polysaccharide extract contained 31.17%
neutral polysaccharides and 38.44% uronic acid and was considered as pectic polysaccharides. The polysaccharides from
Cyclocarya paliurus leaves formed flocculation in in vitro gastric digestion model, while the flocculation was eliminated in in vitro
gastrointestinal model. The molecular weights of digestion products at different digestion times remained unchanged in in vitro
simulated gastric and upper gastrointestinal model. Compared with aqueous solution, the retention time of these polysaccharides
dissolved in the gastric juice was shorter in high performance gel permeation chromatography. Meanwhile, reducing sugar and
free monosaccharide contents of digested products also kept unchanged in simulated gastric and upper gastrointestinal models.
Therefore, the polysaccharides from Cyclocarya paliurus leaves were not digested in vitro, but to some extent, their stability and
configuration were affected by acidic environment in in vitro simulated gastric model and gastric electrolytes, respectively.

Key words: polysaccharides from Cyclocarya paliurus leaves, in vitro simulated upper gastrointestinal model, in vitro digestion, in vitro absorption

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