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Desalination Using Mixed-Bed Ion Exchange Resin and Physiochemical Properties of Grass Carp Peptides

CUI Chen，CHEN Yue，CHEN Ji-wang，XIA Wen-shui   

  1. 1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China；
    2. School of Food Science and Technology, Jiangnan Unviersity, Wuxi 214122, China
  • Online:2013-09-25 Published:2013-09-27

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Abstract:

In the present study, we investigated the process conditions for desalinating grass carp peptides (GCPs) using
mixed-bed ion exchange resin and characterized desalinated GCPs. The optimal desalination conditions for GCPs were
sample concentration of 10 mg/mL, loading flow rate of 6 BV/h, pH 7.0 and anion/cation exchange resin ratio of 2:3. Under
these conditions, the desalination rate was 81.7% and the peptide recovery was 80.5%; meanwhile, the ash content in GCPs
was reduced from 17.5% to 3.2%, and the peptide content was increased from 80.9% to 90.3%. The relative molecular mass
of desalinated GCPs was mostly distributed in the range of 190 to 1000 in an amount of 91.5%, solubility was greater than
95% in the pH range of 2 to 10, and viscosity was only 4.5 mPa•s at the concentration of 40 g/100 mL.

Key words: grass carp peptides, mixed-bed ion exchange resin, physiochemical properties