Abstract:

The optimized separation conditions for α-linolenic acid from pricklyash seed oil were determined by using
single factor experiments and response surface analysis. The ratio of water to β-CD, inclusion temperature and inclusion time
were used as the independent variables and the yield of α-linolenic acid was used as the response value. The results showed
that the optimum separation conditions were the ratio of water to β-CD 9.9:1, inclusion temperature 64 ℃ and inclusion
time 1.32 h. The resulting inclusion rate reached 22.42%. The occurrence of inclusion reactions was verified by FT-IR. The
composition of unsaturated fatty acids was analyzed by GC-MS after inclusion reactions, and three fatty acids were retained
in the oil. The content of α-linolenic acid was improved from 4.12% to 21.35%.

Key words: pricklyash seed oil；&alpha, -linolenic acid；&beta, -cyclodetrin；response surface analysis