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Application of Response Surface Methodology to Optimize Extraction of Protein from Potato Starch Wastewater by Sodium Alginate

LIU Ting-ting,YAO Jia,ZHANG Fei-jun,WANG Da-wei   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Online:2013-09-25 Published:2013-09-27

Abstract:

Sodium alginate was used as a natural polymer flocculant to recover protein from potato starch wastewater.
In addition, the amino acid composition of the recovered protein was analyzed. Box-Behnken design coupled with
response surface analysis was used to optimize four variables for recovering protein including sodium alginate dosage,
pH, temperature and stirring time. The optimal process conditions of the variables were found to be stirring with 0.97 g/L
sodium alginate at pH 3.6 for 35 min at 35 ℃, resulting in a protein recovery of 70.93%. The total amino acid content of the
recovered protein was 69.43% and the ratio of essential amino acids to total amino acids was 40.33%. Tryptophane was the
first limiting amino acid.

Key words: sodium alginate, potato starch, wastewater, protein