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Putrescine-producing Property of Escherichia coli YSY1 and Related Genes

XU Wen-juan1.2,LIU Fang2,WANG Dao-ying2,ZHU Yong-zhi2,ZHOU Tao1,*,XU Wei-min2     

  1. 1. Ginling College, Nanjing Normal University, Nanjing 210097, China;
    2. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: ZHOU Tao

Abstract:

HPLC was used to detect the levels of putrescine and spermine produced by Escherichia coli YSY1, and PCR was
used to amplify decarboxylase gene. The results showed that the level of putrescine was increased during fermentation, but the
level of spermine revealed a decrease at the same time. The content of spermine between 1 h and 6 h of fermentation changed
from 198.04 μg/mL to 156.33 μg/mL, whereas the amount of putrescine varied from 14.61 μg/mL to 18.83 μg/mL. Ornithine decarboxylase
gene was successfully amplified from Escherichia coli genome, while arginine decarboxylase gene and other related genes were not detected.

Key words: biogenic amines, putrescine, spermine, PCR, HPLC

CLC Number: