Abstract:

A novel and stable artificial ractopamine hapten with the active group carboxyl group was designed and
synthesized by chemical modification, and linked to bovine serum albumin through ethyl carbodiimide hydrochloride
method to form artificial ractopamine antigen. The conjugation was confirmed by UV scanning method and protein
electrophoresis. The binding reaction of the artificial ractopamine antigen with anti-ractopamine antibody was verified by
gold immunochromatography assay. The ractopamine hapten was characterized by gas chromatography-mass spectrometry.
The ractopamine-BSA conjugation was stable and active for at least 6 months at 4 ℃. Our results indicate that ractopamine-
BSA can be successfully synthesized by the above method, and can be used as coating antigen in immunoassay for
ractopamine detection. In addition, we found that the synthesized antigen could be used as an immunogen to produce antiractopamine
antibody.

Key words: ractopamine, hapten, artificial antigen, ractopamine, immunoassay